Publication-quality ΔΔCq analysis, interactive figures, and automated quality control. No software to install, no subscription. Your data is never stored on our servers.
Start AnalyzingqPCR Guru replaces $1,500/yr commercial tools — no subscription, no complex setup. Just upload your data and go.
Relative and absolute quantification with primer efficiency correction. Support for multiple reference genes with geNorm M and NormFinder ρ stability ranking.
Automated compliance checklist covering NTC validation, replicate consistency, outlier detection, and reference gene stability — built to the 2025 MIQE 2.0 standard.
Automated experiment interpretation that tells you what your data means — not just what it looks like. Expression overview, quality flags, and suggested follow-up experiments.
Bar charts, heatmaps with hierarchical clustering, PCA, volcano plots, correlation scatter plots, and melt curves — all exportable at 300+ DPI as JPEG, TIFF, or PDF.
Plate heatmaps, replicate SD and CV% flagging, MAD outlier detection, NTC monitoring, and customizable Cq/SD thresholds — all computed instantly on upload.
Multi-page US Letter reports with title page, all figures, MIQE compliance, and Guru Insights. Choose which sections to include. Ready for lab notebooks or publications.
Upload dilution series data for primer efficiency, LOD/LOQ, and copy number estimation. Seamlessly switch between relative and absolute quantification modes.
Auto-detects 7+ instrument formats. Merge multiple files for cross-plate analysis. Inter-run calibrator detection with automatic plate drift assessment.
Full support for the MIQE-endorsed RDML standard. Import from other tools or export your data for seamless collaboration and archival.
See how qPCR Guru compares to commercial alternatives.
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